Use of a hybrid infectivity assay to analyze primary transcription of temperature-sensitive mutants of the New Jersey serotype of vesicular stomatitis virus
نویسندگان
چکیده
منابع مشابه
Isolation and characterization of temperature-sensitive mutants of vesicular stomatitis virus, New Jersey serotype.
Forty-eight temperature-sensitive (ts) mutants have been isolated from a wild-type strain of the New Jersey serotype of vesicular stomatitis virus (VSV) after exposure to the base analogue mutagen 5-fluorouracil. Of these mutants, 47 have been classified into 6 nonoverlapping complementation groups containing 21, 17, 4, 3, 2, and 1 mutant, respectively (1 mutant remaining unallocated). The ribo...
متن کاملTemperature-Sensitive Mutants of Vesicular Stomatitis Virus
Different temperature-sensitive mutants of vesicular stomatitis virus have been characterized in terms of their ability to induce synthesis of viral ribonucleic acid (RNA) in BHK-21 cells at 39 C (the restrictive temperature for these mutants). Mutants belonging to complementation groups I and IV (and probably II) did not induce actinomycin-resistant RNA synthesis in infected cells incubated at...
متن کاملPrimary in vivo transcription of vesicular stomatitis virus and temperature-sensitive mutants of five vesicular stomatitis virus complementation groups.
The process of adsorption and RNA transcription by vesicular stomatitis virus (VSV) in cells has been followed by using highly labeled preparations of VSV virions. The initial transcription process (primary parental transcription) is rapid and takes about 4 min to develop complete transcripts of the input genome. The optimal cell temperature for in vivo transcription is between 36 and 39.5 C, a...
متن کاملTemperature-sensitive mutants of vesicular stomatitis virus; homology and nomenclature.
Reciprocal complementation experiments in mouse L cells and BHK-2I cells show that the complementing temperature-sensitive (ts) mutants of vesicular stomatitis virus (VSV), Indiana serotype, isolated from the HR wild-type strain, belong to groups I, III and IV (Flamand & Pringle, I97I). Since the homologies of ts mutants isolated from three different wild-type strains growing in different host ...
متن کاملStudy of the transcription and the replication of vesicular stomatitis virus by using temperature-sensitive mutants.
The viral ribonucleic acids (RNA species) synthesized in HeLa cells infected with temperature-sensitive (ts) mutants and with the wild type of vesicular stomatitis virus at permissive (30 C) and nonpermissive (39.2 C) temperatures were compared by sucrose gradient centrifugation. Two ts mutants (ts 5 and ts 100) representing two separate complementation groups (respectively, groups I and IV), e...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Virology
سال: 1982
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.43.1.37-40.1982